Application

This Anti-ABCA1 Antibody, clone AB.H10 is validated for use in ELISA, IP, WB, IH(P) for the detection of ABCA1.

ELISA
Immunohistochemistry (Paraffin): 5 µg/mL. Perform heat mediated antigen retrieval in sodium citrate buffer (pH 6), before commencing with IHC staining protocol. Amplification with biotin-streptavidin may be required.
Immunoprecipitation
Western Blot: 1 µg/mL. Predicted molecular weight: 254 kDa.

Optimal working dilutions must be determined by end user.

Research CategoryMetabolism

Research Sub CategoryLipid Metabolism & Weight Regulation

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Mutations in the ABCA1 gene are associated with Tangier disease (TD). TD is an autosomal recessive disorder results from an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and disorders in cellular lipid trafficking. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI.

Immunogen

Recombinant fragment, corresponding to amino acids 1800-2260 of Human ABCA1.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Presented as a liquid in PBS, pH 7.4 with 0.05% sodium azide.

Specificity

Recognize specifically ABCA1 from human, mouse, and chicken.

Storage and Stability

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4°C for 3 months.